english
français
RP2E INRA Université de Lorraine

Publications

In vitro follicle culture shows that progestagens are the maturation-inducing hormones (MIH) and possible regulators of the ovulation-mediating hormone PGE2 in female Eurasian perch Perca fluviatilis

Fish Physiology and Biochemistry, 47(4), 881-894

El Mohajer, L., Bulteau, R., Chevalier, C., Selmi, S., Fontaine, P., Milla, S.

2021

In European aquaculture, Eurasian perch, Perca fluviatilis L., is perceived as one of the most highly valuable freshwater fish species and a strong candidate for the development of freshwater aquaculture. In the pursuit of improving the quality of reproduction in this domesticated species, investigating the hormones mediating the Final Oocyte Maturation (FOM) is therefore indispensable. But, the exact nature of the Maturation Inducing Hormone (MIH) in Eurasian perch is unknown. To further validate the existence of a maturation inducing activity behind potential hormonal candidates in this species, we in vitro tested a group of nine hormones; cortisol (Co), 11-deoxycortisol (11-D), corticosterone (coS), 11-deoxycorticosterone (DOC), 17α,20βdihydroxy-4-pregnen-3-one (DHP) and 17α,20β,21 trihydroxy-4-pregnen-3-one (THP), prostaglandinE2 (PGE2), estradiol-17β (E2) and testosterone (T), in their ability to trigger FOM advancement and the production of sex steroids potentially involved in FOM. Using mature female perch, two in vitro experiments were conducted with oocytes at the start of the FOM. The follicles were incubated for 62 hours in Cortland media with and without human chorionic gonadotrophin (hCG). By the end of the incubation, only DHP and THP triggered the full advancement in FOM even at low doses with the effect of DHP being in vivo validated. However, the de novo productions of E2 and DHP were not shown to be regulated by either of the MIH candidates. Progestagens are hence more credible candidates as MIH than corticosteroids in Eurasian perch. Our in vitro study also revealed that both PGE2 and DHP are strongly associated with ovulation and that PGE2 might have slightly contributed to such DHP activity.

Imprimer le document

Voir le document sur le site de l'éditeur

179 Publications depuis 2019

Imprimer le listing

2024

ACL - Articles comité de lecture référencés bases de données HCÉRES

2023

ACL - Articles comité de lecture référencés bases de données HCÉRES

2022

ACL - Articles comité de lecture référencés bases de données HCÉRES

2021

ACL - Articles comité de lecture référencés bases de données HCÉRES

ACLN - Articles comité de lecture non-référencés bases de données HCÉRES

2020

ACL - Articles comité de lecture référencés bases de données HCÉRES

ACLN - Articles comité de lecture non-référencés bases de données HCÉRES

2019

ACL - Articles comité de lecture référencés bases de données HCÉRES

ACLN - Articles comité de lecture non-référencés bases de données HCÉRES

Voir les productions antérieures

Toutes les productions scientifiques