english
français
RP2E INRA Université de Lorraine

Publications

Influence of domestication process on immune response to repeated emersion stressors in Eurasian perch (Perca fluviatilis, L.)

Comp. Biochem. Physiol. Part A, 173, pp. 52-60.

Douxfils, J., Lambert, S., Mathieu, C., Milla, S., Mandiki, R., Henrotte, E., Henrotte, E., Wang, N., Dieu, M., Rougeot, C., Kestemont, P.

2014

Domestication might be a possible way to reduce the physiological response to long-term stressors and deleterious effects on immunity. The present study aimed to evaluate the chronic immune response induced by repeated emersions and the possible impact of domestication by comparing farmed Eurasian perch with short (F1) and long (F4) captive-life history. In the first experiment, fish were exposed to a single emersion and physiological stress response was measured in the short term to characterize fish sensitivity to the tested stressor. Serum cortisol and glucose elevated within 6h post-stress and splenosomatic index (SSI) decreased within 48h, indicating that the species was affected by emersion stressor. In the second experiment, F1 and F4 generations were submitted to repeated water emersions (3 times/week during 44days). On day 9, 18 and 44, samplings were performed 48h post-stressor to highlight any sustained disruption of immune system. Serum cortisol, glucose, SSI and lysozyme activity were evaluated and serum proteome was analyzed using 2D-DIGE. Any of the tested variables were affected by repeated emersions and proteomic analysis only revealed that alpha-2 macroglobulins (a2Ms) were up-regulated in the serum of stressed individuals. Domestication also resulted in the up-regulation of five a2M isoforms and down-regulation of complement C3 and Ig light chain proteins, independently of any stressor exposure. In conclusion, the results suggested that repeated emersions are not severe stressors for Eurasian perch, probably explaining why domestication had no influence on fish responses. Changes associated with domestication are highly complex and certainly need further investigations

Imprimer le document

178 Publications depuis 2013

Imprimer le listing

2018

ACL - Articles comité de lecture référencés bases de données AERES

2017

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

2016

ACL - Articles comité de lecture référencés bases de données AERES

2015

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

2014

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

2013

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

Voir les productions antérieures

Toutes les productions scientifiques