english
français
RP2E INRA Université de Lorraine

Publications

Modifications of the endosomal compartment in peripheral blood mononuclear cells and fibroblasts from Alzheimer's disease patients

Transl Psychiatry., 5, e595.

Corlier, F., Rivals, I., Lagarde, J., Hamelin, L., Corne, H., Dauphinot, L., Ando, K., Cossec, J.-C., Fontaine, G., Dorothée, G., Malaplate-Armand, C., Olivier, J.-L., Dubois, B., Bottlaender, M., Duyckaerts, C., Sarazin, M., Potier, M.-C., ImaBio3

2015

Identification of blood-based biomarkers of Alzheimer's disease (AD) remains a challenge. Neuropathological studies have identified enlarged endosomes in post-mortem brains as the earliest cellular change associated to AD. Here the presence of enlarged endosomes was investigated in peripheral blood mononuclear cells from 48 biologically defined AD patients (25 with mild cognitive impairment and 23 with dementia (AD-D)), and 23 age-matched healthy controls using immunocytochemistry and confocal microscopy. The volume and number of endosomes were not significantly different between AD and controls. However, the percentage of cells containing enlarged endosomes was significantly higher in the AD-D group as compared with controls. Furthermore, endosomal volumes significantly correlated to [C(11)]PiB cortical index measured by positron emission tomography in the AD group, independently of the APOE genotype, but not to the levels of amyloid-beta, tau and phosphorylated tau measured in the cerebrospinal fluid. Importantly, we confirmed the presence of enlarged endosomes in fibroblasts from six unrelated AD-D patients as compared with five cognitively normal controls. This study is the first, to our knowledge, to report morphological alterations of the endosomal compartment in peripheral cells from AD patients correlated to amyloid load that will now be evaluated as a possible biomarker.

Imprimer le document

179 Publications depuis 2013

Imprimer le listing

2018

ACL - Articles comité de lecture référencés bases de données AERES

2017

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

2016

ACL - Articles comité de lecture référencés bases de données AERES

2015

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

2014

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

2013

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

Voir les productions antérieures

Toutes les productions scientifiques