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Mémoire de Master 2ème année mention BAAN, spécialité SPBA
Uriot, O.
2012
Streptococcus thermophilus is a lactic acid bacterium widely used in the dairy industry and is therefore frequently found in the gastric intestinal tract (GIT) of fermented-product consumers. While beneficial properties to human health are recognized for S. thermophilus like lactose intolerance improvement, its probiotic status is still questioned.
In this project, we aimed at better understanding the survival capacities and the physiology of S. thermophilus during its passage in the GIT.
The survival capacities of the two strains PB18O and LMD9 were assessed in the compartments of the TIM system, an in vitro model simulating the human upper part of the digestive tract. Both strains delivered in milky meal were found to sensitive to the acidic pH of the stomach and to the contact of bile salts in the small intestine.
In the second part of the study, the R-IVET (Recombinase-based in vivo Expression Technology) strategy was used to investigate the metabolic activity of S. thermophilus LMD9 in the GIT. In the gastric compartment of the TIM, two genes were found to be active, STER_1905 (hisS) responsible for the synthesis of histidyl-transfer RNA and STER_1406 encoding the component of an ABC-type oligopeptide / nickel transport system.
soutenue le 5 décembre 2023
Delannoy, M.
soutenue le 1er décembre 2023
Fournier, A.
Lecocq, T.
Hardy, A.; Pasquet. A.; Diakos, E., Lecocq, T.
Praion, M.
Amoussou, N., Lecocq, T., Thomas, M.
Amutova, F.
El Mohajer, L.
Vigier, M.
Bled, A., Pasquet, A., Lecocq, T.
Hardy, A., Lecocq, T., Pasquet, A., Thomas, M.
15 décembre 2021
El Wanny, N.
Le Cor, F.
Butruille, G., Thomas, M., Lecocq, T.
Chevalier, C.
El manfaloti, M.A., Thomas, M., Pasquet, A., Lecocq, T.
Cakir-Kiefer, C.
soutenue le 16 décembre 2020
Fourcot, A.
Kuznetcova, D.
Toomey, L., Fontaine, P., Lecocq, T.
Bulteau, R.
El Hajj, A.
Rocha De Almeida, T.
Ado Kanta, H.
Gouret, M., Toomey, L., Pasquet, A., Lecocq, T.
Reynaud, J-G., Lecocq, T., Pasquet, A., Thomas, M.
Schaeffer, L., Lecocq, T., Pasquet, A., Thomas, M.
Speder, K., Toomey, L., Lecocq, T.