ß-sitoglucoside saponin was separated from Anabasis articulata: plant used in traditional medicine for treatment of diabetes (Chenopodiaceae). Different methods and spectroscopic chromatography have allowed to identify the product isolated. Experiments were performed in non-diabetic mice, and in diabetic mice (glucose treated mice) showed that oral administration of the butanolic extract (saponins) 10 mg/kg decreased the glycaemia to 20.09% (P < 0.05), six hours after administration. These results also showed that oral administration of 4 mg/kg of ß-Sitoglucoside decrease the glycaemia to 24.2% (P < 0.05) after six hours of treatment and corresponding to the greatest reduction in diabetic mice (anti-hyperglycemic effect) similar to the antidiabetic action of reference molecule: glibenclamide (10 mg/kg). This dose has been able to restore the blood glucose level in diabetic mice, whereas the concentration of 2 mg/kg had no appreciable effect. This study confirms the effect of the antidiabetic compound separated.