Garcinia cambogia extract (GCE) is a popular weight-loss supplement that also lowersplasma triglyceride (TG) levels. We hypothesized that GCE-mediated inhibition of ATP citrate lyaseand thereby hepatic TG production could lead to compensatory mechanisms, including increasedhepatic TG uptake via lipoprotein receptors. GCE (20 mg/day) administered 40 days orally to femaleC57BL/6Rj mice on a standard chow diet led to a decrease in both plasma fasting and post-prandialTG-rich lipoprotein levels, but with no significant change in body weight gain. Lipolysis stimulatedlipoprotein receptor (LSR) protein levels, but not those of LDL-receptor, were increased as comparedto controls. Mouse Hepa1-6 cells treated with the GCE active ingredient, hydroxycitrate, also led toincreased LSR protein levels. Hepatic total cholesterol, TG, and muscle TG contents were higher inGCE-treated animals as compared to controls, whereas adipose TG levels were unchanged. LSR andLDL-receptor protein levels were correlated with liver total cholesterol, but only LDL-receptor wasassociated with liver TG. These results show that GCE treatment in mice on a standard chow diet ledto significantly increased liver and muscle lipids, with no significant change in adipose tissue TGlevels, which should be considered in the long-term use of GCE