english
français
RP2E INRA Université de Lorraine

Publications

Cerebrospinal fluid Abeta42/Abeta40 as a means to limiting tube- and storage-dependent pre-analytical variability in clinical setting

J Alzheimers Dis, 57 (2), pp. 437-445.

Gervaise-Henry, C., Watfa, G., Albuisson, E., Kolodziej, A., Dousset, B., Olivier, J.-L., Jonveaux, T.-R., Malaplate-Armand, C.

2017

BACKGROUND:

Alzheimer's disease (AD) cerebrospinal fluid (CSF) biomarkers have recently been included in the criteria for AD diagnosis. Unfortunately, their wider use in routine and interpretation require more standardization, particularly for the pre-analytical steps. In particular, amyloid-β (Aβ)42 peptide measurement is strongly influenced by the type of collection tube and by repeated freeze/thaw cycles.

OBJECTIVE:

The objectives of this study were to compare, the in clinical setting, the impact of collection tubes and the repetition of freeze/thaw cycles on Aβ42 and Aβ40 concentrations and consequently determine if the Aβ42/Aβ40 ratio could resolve the diagnosis difficulties related to these pre-analytical parameters.

METHODS:

CSF from 35 patients was collected in different polypropylene (PP) and stored at - 80°C after sampling. For CSF collected in the reference tube, three successive freeze-thaw cycles were done. Aβ42 and Aβ40 concentrations were determined in each condition in order to calculate the Aβ42/Aβ40 ratio.

RESULTS:

Our results showed that CSF Aβ42 and Aβ40 values were significantly different according to the type of collection tube and the number of freeze/thaw cycles. Although the calculation of the Aβ42/Aβ40 ratio eliminated the effect of PP tube-dependent variation, this was not the case for freeze-thaw cycle-associated variation.

CONCLUSION:

The use of Aβ42/Aβ40 ratio rather than Aβ42 alone could contribute toward pre-analytical standardization, thus allowing the general use of CSF AD biomarkers in routine practice.

Imprimer le document

202 Publications depuis 2013

Imprimer le listing

2018

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

  • Humusica: Soil biodiversity and global change

    Bulletin of geography: Physical Geography Series, 14 (1), pp. 15-36.

    Zanella, A., Ascher-jenull, J., Ponge, J.F., Bolzonella, C., Banas, D., De nobili, M., Fusaro, S., Sella, L., Giannini, R.

2017

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

2016

ACL - Articles comité de lecture référencés bases de données AERES

2015

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

2014

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

2013

ACL - Articles comité de lecture référencés bases de données AERES

ACLN - Articles comité de lecture non-référencés bases de données AERES

Voir les productions antérieures

Toutes les productions scientifiques