english
français
RP2E INRA Université de Lorraine

Publications

Brain region-specific immunolocalization of the lipolysis-stimulated lipoprotein receptor (LSR) and altered cholesterol distribution in aged LSR+/- mice

J Neurochem, 123 (4), pp. 467-476.

Stenger, C., Pinçon, A., Hanse, M., Royer, L., Koziel, V., Comte, A., Olivier, J.-L., Pillot, T., Yen, F.T.

2012

Brain lipid homeostasis is important for maintenance of brain cell function and synaptic communications, and is intimately linked to age-related cognitive decline. Because of the blood–brain barrier’s limiting nature, this tissue relies on a complex system for the synthesis and receptor-mediated uptake of lipids between the different networks of neurons and glial cells. Using immunofluorescence, we describe the region-specific expression of the lipolysis-stimulated lipoprotein receptor (LSR), in the mouse hippocampus, cerebellum Purkinje cells, the ependymal cell interface between brain parenchyma and cerebrospinal fluid, and the choroid plexus. Colocalization with cell-specific markers revealed that LSR was expressed in neurons, but not astrocytes. Latency in arms of the Y-maze exhibited by young heterozygote LSR+/ mice was significantly different as compared to control LSR+/+, and increased in older LSR+/ mice. Filipin and Nile red staining revealed membrane cholesterol content accumulation accompanied by significantly altered distribution of LSR in the membrane, and decreased intracellular lipid droplets in the cerebellum and hippocampus of old LSR+/ mice, as compared to control littermates as well as young LSR+/ animals. These data therefore suggest a potential role of LSR in brain cholesterol distribution, which is particularly important in preserving neuronal integrity and thereby cognitive functions during aging.

Imprimer le document

178 Publications depuis 2019

Imprimer le listing

2024

ACL - Articles comité de lecture référencés bases de données HCÉRES

2023

ACL - Articles comité de lecture référencés bases de données HCÉRES

2022

ACL - Articles comité de lecture référencés bases de données HCÉRES

2021

ACL - Articles comité de lecture référencés bases de données HCÉRES

ACLN - Articles comité de lecture non-référencés bases de données HCÉRES

2020

ACL - Articles comité de lecture référencés bases de données HCÉRES

ACLN - Articles comité de lecture non-référencés bases de données HCÉRES

2019

ACL - Articles comité de lecture référencés bases de données HCÉRES

ACLN - Articles comité de lecture non-référencés bases de données HCÉRES

Voir les productions antérieures

Toutes les productions scientifiques